品牌:美国 NewEast Biosciences
货号: 26163
抗原:Synthetic peptide
规格:100 微升
产品全名:Anti-IDH2(R172K) Mouse Monoclonal Antibody
应用: ELISA, WB, IF, IHC
种属反应性的选择:Human, Mouse and Rat
物种/宿主:Mouse
描述: Anti-IDH2(R172K) Mouse Monoclonal Antibody
背景介绍: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas.
免疫原: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin
经过测试的应用: ELISA, WB, IHC
推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IHC: 1: 50-1: 100 IHC: 1: 10-1: 100
浓度: 1 mg/ml
宿主: Mouse
形式: Liquid
克隆性: Monoclonal
亚型: IgG
纯度: Purified from ascites
防腐剂: No
成分: PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性: recognizes R172K mutant, but not wild-type IDH2 of vertebrates
储存条件: Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:
Western blot analysis of recombinant IDH2(R172K) and wildtype proteins. Purified His-tagged IDH2(R172K) (lane 2) and wildtype protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (Cat. #26163). 免疫荧光:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody. HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #26163).
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处，也是目前所有产品国内经销处。
目前客户使用产品发表超过1000谷歌学术文献，其中论文分布排名前50刊物上。
NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体**的信号转导，包括跨膜受体，例如介导味觉、嗅觉和视觉的那些，（2）核糖体的蛋白质生物合成，（3）调节细胞分化、增殖、分裂和运动，（4）蛋白质通过膜的易位，（5）细胞内囊泡的运输，以及囊泡介导的分泌和摄取，通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
更多产品类目和技术资料可查看：武汉费斯德生物官网。
