品牌:美国 NewEast Biosciences
货号: 26904
抗原:Full Recombinant active RhoA
规格:30 微升
产品全名:Anti-RhoA-GTP Mouse Monoclonal Antibody
应用: IP, IHC, IF
种属反应性的选择:Human, Mouse and Rat
物种/宿主:Mouse
描述: Anti-RhoA-GTP Mouse Monoclonal Antibody
背景介绍: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
免疫原: Recombinant full length protein of active RhoA
经过测试的应用: IP, IHC,IF
推荐稀释比: IP: 1 μg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
浓度: 1 mg/ml
宿主: Mouse
形式: Liquid
克隆性: Monoclonal
亚型: IgG2b
纯度: Purified from ascites
防腐剂: No
成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性: Anti-RhoA monoclonal antibody recognizes active RhoA from vertebrates.
储存条件: Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western Blot:
IP/WB Analysis of Active RhoA Protein.Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-RhoA-GTP monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26007).
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处，也是目前所有产品国内经销处。
目前客户使用产品发表超过1000谷歌学术文献，其中论文分布排名前50刊物上。
NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体**的信号转导，包括跨膜受体，例如介导味觉、嗅觉和视觉的那些，（2）核糖体的蛋白质生物合成，（3）调节细胞分化、增殖、分裂和运动，（4）蛋白质通过膜的易位，（5）细胞内囊泡的运输，以及囊泡介导的分泌和摄取，通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
更多产品类目和技术资料可查看：武汉费斯德生物官网。
